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Aislamiento y cultivo de células de la cresta neural de embriones murinos del Tubo Neural
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
DOI:

12:48 min

June 02, 2012

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Chapters

  • 00:05Title
  • 01:14Introduction
  • 01:36Prepare Plates
  • 02:18Preparation of Self Renewal and Wash Media
  • 03:06Preparation of Collagenase/Dispase
  • 04:22Isolating Vagal and Trunk Neural Tube
  • 08:21Removal of Non-Neural Ectoderm and Mesoderm
  • 11:06Removal of the Neural Tube
  • 11:46Representative Results
  • 12:35Conclusions

Summary

Automatic Translation

Aislamiento de cresta neural embrionario del tubo neural facilita el uso de<em> In vitro</em> Métodos para el estudio de la migración, la auto-renovación, y multipotencia de la cresta neural.

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