Journal
/
/
Isolamento e cultivo de células da crista neural do tubo neural embrionário murino
JoVE Journal
Neuroscience
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
DOI:

12:48 min

June 02, 2012

, ,

Chapters

  • 00:05Title
  • 01:14Introduction
  • 01:36Prepare Plates
  • 02:18Preparation of Self Renewal and Wash Media
  • 03:06Preparation of Collagenase/Dispase
  • 04:22Isolating Vagal and Trunk Neural Tube
  • 08:21Removal of Non-Neural Ectoderm and Mesoderm
  • 11:06Removal of the Neural Tube
  • 11:46Representative Results
  • 12:35Conclusions

Summary

Automatic Translation

Isolamento de crista neural embrionário a partir do tubo neural facilita o uso de<em> In vitro</em> Métodos para estudar a migração, a auto-renovação, e multipotency da crista neural.

Related Videos

Read Article