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Isolering och odling av neurallistceller från embryonala Murine Neural Tube
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
DOI:

12:48 min

June 02, 2012

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Chapters

  • 00:05Title
  • 01:14Introduction
  • 01:36Prepare Plates
  • 02:18Preparation of Self Renewal and Wash Media
  • 03:06Preparation of Collagenase/Dispase
  • 04:22Isolating Vagal and Trunk Neural Tube
  • 08:21Removal of Non-Neural Ectoderm and Mesoderm
  • 11:06Removal of the Neural Tube
  • 11:46Representative Results
  • 12:35Conclusions

Summary

Automatic Translation

Isolering av embryonala neurallisten från det neurala röret underlättar användningen av<em> In vitro</em> Metoder för att studera migration, självförnyelse och multipotens av neural crest.

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