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恒压控制的挤压方法制备纳米脂质体
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Bioengineering
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JoVE Journal Bioengineering
Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles
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恒压控制的挤压方法制备纳米脂质体

DOI:
10.3791/4151-v

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11:35 min

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June 22, 2012

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, ,
1Department of Chemistry & Biochemistry,University of Colorado Boulder, 2Biofrontiers Institute,University of Colorado Boulder

Chapters

  • 00:05Title
  • 01:13Liposome Preparation
  • 02:46Extrusion
  • 05:28Dynamic Light Scattering (DLS) Analysis
  • 06:18Nanoparticle Tracking Analysis (NTA)
  • 07:35Results: Analysis of Nano-sized Lipid Vesicles
  • 11:06Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了一个准备一个高度同质化的亚微米大小的脂质体的挤压方法。此方法使用脂质体的制备与控制氮流量压力控制系统。脂质准备<sup> 1,2</sup>，脂质体挤压，大小特征将在此呈现。

Tags

Pressure-controlled Extrusion MethodNano-sized Lipid VesiclesLiposomesPhospholipidsProtein-protein InteractionsProtein-lipid InteractionsDrug DeliveryEncapsulationCurved Lipid BilayersLarge Unilamellar Vesicles (LUVs)Small Unilamellar Vesicles (SUVs)Multilamellar Vesicles (MLVs)Membrane CurvatureCell SignalingEndo- And ExocytosisMembrane FusionProtein TraffickingLiposome-drug EncapsulationDrug EncapsulationExtrusion Step

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