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定量,实时分析,利用分子信标病变特定的细胞裂解液中的碱基切除修复活动
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Biology
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JoVE Journal
Biology
Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
Please note that all translations are automatically generated.
Click here for the English version.
定量,实时分析,利用分子信标病变特定的细胞裂解液中的碱基切除修复活动
DOI:
10.3791/4168-v
•
15:01 min
•
August 06, 2012
•
David Svilar
2
,
Conchita Vens
,
Robert W. Sobol
2,4
1
Department of Pharmacology & Chemical Biology
,
University of Pittsburgh School of Medicine
,
2
Hillman Cancer Center
,
University of Pittsburgh Cancer Institute
,
3
Department of Experimental Therapy
,
The Netherlands Cancer Institute
,
4
Department of Human Genetics
,
University of Pittsburgh School of Public Health
Chapters
00:05
Title
02:53
Molecular Beacon Design
04:31
Nuclear Lysate Preparation
08:31
Molecular Beacon Assay
11:51
Representative Analysis of Tumor Cell Lysates
13:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了DNA糖基化酶和AP内切酶的活动,在核细胞裂解定量,实时测量的方法。该法产生服从动力学分析DNA修复活动率,是适应组织和肿瘤裂解物或纯化蛋白质的DNA修复能力的量化。
Tags
Quantitative Analysis
Real-time Measurement
Base Excision Repair
DNA Glycosylase
AP Endonuclease
Molecular Beacons
Substrate
Deoxyoligonucleotide
Carboxyfluorescein (6-FAM)
Dabcyl
Stem-loop Structure
Nucleotides
Base Pairs
Förster Resonance Energy Transfer (FRET)
Fluorescence
DNA Repair
Fluorescence Values
Real-time Assessment
PCR Instruments
Article
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