Biology
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Cuantitativo, análisis en tiempo real de la actividad de reparación por escisión de base en lisados celulares que utilizan la lesión específicos Molecular beacons
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Summary August 6th, 2012
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Se describe un método para la determinación cuantitativa, medida en tiempo real de ADN glicosilasa y actividades AP endonucleasas en lisados de células nucleares. El ensayo proporciona tasas de actividad ADN Reparación susceptibles de análisis cinético y es adaptable para la cuantificación de la actividad del ADN en el tejido de reparación y lisados tumorales o con proteínas purificadas.
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