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JoVE Journal Biology
Kvantitativ, Real-time analyse af base excision reparation aktivitet i cellelysater Udnyttelse Læsionssynligheden-specifikke Molecular Beacons
 
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Kvantitativ, Real-time analyse af base excision reparation aktivitet i cellelysater Udnyttelse Læsionssynligheden-specifikke Molecular Beacons

Article doi: 10.3791/4168
August 6th, 2012 •
1,2, 3, 1,2,4
1Department of Pharmacology & Chemical Biology, University of Pittsburgh School of Medicine, 2Hillman Cancer Center, University of Pittsburgh Cancer Institute, 3Department of Experimental Therapy, The Netherlands Cancer Institute, 4Department of Human Genetics, University of Pittsburgh School of Public Health

Summary
Automatic Translation
August 6th, 2012

Please note that all translations are automatically generated.

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Beskriver vi en fremgangsmåde til kvantitativ real-time måling af DNA-glycosylase og AP-endonuclease aktiviteter cellekerner lysater. Assayet giver hastigheder af DNA-reparation aktivitet kan underkastes kinetisk analyse og kan tilpasses til kvantificering af DNA-reparation aktivitet i væv og tumorer lysater eller oprensede proteiner.

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