Journal
/
Biology
/
糖基谱研究植物细胞壁聚合物，用微阵列
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Glycan Profiling of Plant Cell Wall Polymers using Microarrays
Please note that all translations are automatically generated. Click here for the English version.

糖基谱研究植物细胞壁聚合物，用微阵列

DOI:
10.3791/4238-v

•

12:30 min

•

December 17, 2012

•

, , , , ,
1Australian Centre of Excellence in Plant Cell Walls, School of Botany,University of Melbourne, 2Plant Cell Biology Research Centre, School of Botany,University of Melbourne, 3CSIRO Plant Industry, Black Mountain Laboratories, 4Department of Plant Biology and Biotechnology,University of Copenhagen

Chapters

  • 00:05Title
  • 01:40Tissue Collection & Preparation
  • 03:01Extraction of Cell Wall Glycans
  • 04:20Printing Microarrays
  • 05:45Probing of Glycan Microarrays
  • 06:36Quantification
  • 07:49Results: CoMPP Profiling of N. alata
  • 11:17Conclusion

Summary

Automatic Translation

Automatic Translation

一种称为<strong> C</strong> omprehensive<strong> M</strong> icroarray<strong> P</strong> olymer探查（CoMPP）植物细胞壁聚糖的特性进行说明。该方法结合了与一个微型芯片分析平台允许筛选聚糖发生在一个广泛的范围内的生物上下文定义的聚糖-抗原决定簇特异性的单克隆抗体。

Tags

Glycan ProfilingPlant Cell Wall PolymersMicroarraysHeterogeneous GlycansBiosynthesisFunctionGlycosyl ResiduesLinkagesRing StructureIsomeric ConfigurationAnomeric ConfigurationNon-sugar ResiduesDevelopmentEnvironmental CuesGenesArabidopsisReverse Genetics Approaches

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4238?article_type=v

使用微阵列的细菌基因表达分析
check_url/4238?article_type=v

酸性α-膨大诱导测量植物细胞壁扩展（蠕变）
check_url/4238?article_type=v

调查组织和器官特定的光敏色素反应，使用流式细胞仪辅助细胞类型特定的表达谱拟南芥</em
check_url/4238?article_type=v

（充电）酰化tRNA的使用微阵列技术水平的全基因组分析
check_url/4238?article_type=v

胞外多糖的OLIGO质谱分析（OLIMP）
check_url/4238?article_type=v

利用同位素标记的质谱剖析巯基氧化还原蛋白质
check_url/4238?article_type=v

组织化学染色<em>拟南芥</em>次生壁元素
check_url/4238?article_type=v

结晶纤维素积累高分辨率的量化<em>拟南芥</em>根监视特定组织细胞壁修改
check_url/4238?article_type=v

激光辅助显微切割（LAM）作为个体细胞类型的转录谱的工具
check_url/4238?article_type=v

植物多聚核糖体剖面的简单方法

Read Article