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Chapters
- 00:05Title
- 01:34Engineering of Cells with Visible Plasmids
- 05:08Visualization of Labeled DNA
- 09:33Post-acquisition Processing of Images
- 10:33Results: Visualization of Plasmids in Live Mammalian Cells
- 12:16Conclusion
Fremgangsmåte for å observere individuelle DNA molekyler i levende celler er beskrevet. Teknikken er basert på bindingen av en fluorescensmerket tagget lac repressor protein til bindingsseter konstruert inn i DNA av interesse. Denne metoden kan være tilpasset for å følge mange rekombinante DNA'er i levende celler over tid.
Tags
Plasmid ReplicationLive Mammalian CellsFluorescence MicroscopyGamma-herpesvirusesEpstein-Barr VirusKaposi’s Sarcoma-associated HerpesvirusLicensed ReplicationViral ProteinCellular Replication MachineryCentromeresSouthern BlottingFluorescence In Situ Hybridization (FISH)Quantitative PCRVisualizing PlasmidsLactose Repressor ProteinLacO Sequence
