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解剖，文化与分析<em>非洲爪蟾</em>胚胎视网膜组织
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Neuroscience
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JoVE Journal Neuroscience
Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
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解剖，文化与分析<em>非洲爪蟾</em>胚胎视网膜组织

DOI:
10.3791/4377-v

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10:22 min

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December 23, 2012

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, , , , ,
1Department of Biology,College of William and Mary

Chapters

  • 00:05Title
  • 01:26Dissection
  • 06:41Dissociation of Tissue and Plating of Cells
  • 08:24Results: Analysis of Xenopus laevis Embryonic Retinal Tissue
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

非洲爪蟾提供了一个理想的模型系统，为研究细胞命运和生理功能的个人视网膜细胞的原代细胞培养。在这里，我们提出了一种技术，用于解剖视网膜组织并产生成像的原代细胞培养活性钙和原位杂交分析。

Tags

DissectionCultureAnalysisXenopus LaevisNeural PlateVertebrate RetinaClinical ResearchBasic ResearchRetinal DiseaseNeuronal Cell Type DeterminationDifferentiationGanglion CellsAmacrine CellsHorizontal CellsPhotoreceptorsBipolar CellsMüller Glial CellsLayered StructurePrimary Cell CulturePresumptive Retinal CellsDevelopmental StagesCell Fate Specification

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