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Disseksjon, kultur, og analyse av<em> Xenopus laevis</em> Embryonic Retinal Tissue
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Neuroscience
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JoVE Journal Neuroscience
Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue

Disseksjon, kultur, og analyse av<em> Xenopus laevis</em> Embryonic Retinal Tissue

DOI:
10.3791/4377-v

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10:22 min

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December 23, 2012

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, , , , ,
1Department of Biology,College of William and Mary

Chapters

  • 00:05Title
  • 01:26Dissection
  • 06:41Dissociation of Tissue and Plating of Cells
  • 08:24Results: Analysis of Xenopus laevis Embryonic Retinal Tissue
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

Xenopus laevis gir en ideell modell system for å studere cellen skjebne spesifikasjon og fysiologiske funksjon av individuelle netthinnens celler i grunnskolen cellekultur. Her presenterer vi en teknikk for å dissekere retinal vev og generere primære cellekulturer som er avbildet for kalsium aktivitet og analysert ved in situ hybridisering.

Tags

DissectionCultureAnalysisXenopus LaevisNeural PlateVertebrate RetinaClinical ResearchBasic ResearchRetinal DiseaseNeuronal Cell Type DeterminationDifferentiationGanglion CellsAmacrine CellsHorizontal CellsPhotoreceptorsBipolar CellsMüller Glial CellsLayered StructurePrimary Cell CulturePresumptive Retinal CellsDevelopmental StagesCell Fate Specification

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