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一个高含量的成像流程研究Grb2的信号复合体的表达克隆
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
Please note that all translations are automatically generated.
Click here for the English version.
一个高含量的成像流程研究Grb2的信号复合体的表达克隆
DOI:
10.3791/4382-v
•
10:52 min
•
October 30, 2012
•
Jamie Freeman
,
Janos Kriston-Vizi
,
Brian Seed
,
Robin Ketteler
1
MRC LMCB
,
University College London
,
2
Center for Computational and Integrative Biology
,
Massachusetts General Hospital
Chapters
00:05
Title
01:27
Preparation of cDNA Expression Library
05:43
Cell Seeding and Transfection
07:10
Image Acquisition
08:51
Results: Changes in Grb2 Localization by cDNA Expression
10:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
高内涵筛选新的信号主管跨膜受体的鉴定方法。此方法是适合于大规模的自动化,并允许的预测<em在体内</em>蛋白结合和在哺乳动物细胞中的蛋白复合物的亚细胞定位。
Tags
High-content Imaging
Grb2 Signaling Complexes
Expression Cloning
Signal Transduction
Growth Factor Receptors
Downstream Signaling Cascades
Mitogenic Signaling
SH2 Domain
SH3 Domain
GTPase Son-Of-Sevenless (SOS)
Phosphorylated Tyrosine Residues
SOS-Ras-MAP Kinase Signaling Cascade
Positive And Negative Regulator Of Signaling
Receptor Endocytosis
Microscopy Assay
Plasma Membrane Receptors
Epidermal Growth Factor Receptor (EGFR)
GFP-Grb2
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