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Dois registros eletrofisiológicos de sinapticamente evocadas Respostas astroglial e neuronal em fatias hipocampais agudas
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Chapters
- 00:00Title
- 03:58Dissection
- 10:23Dual Recordings of Neuronal fEPSP and Single Astrocytic Whole-cell Response
- 12:07Dual Whole-cell Recordings of Astrocyte and Neuron
- 13:53Representative Results
- 15:45Conclusion
A preparação de fatias cerebrais agudas de isolado hipocampo, assim como as gravações simultâneas electrofisiológicas de astrócitos e neurónios em<em> Stratum radiatum</em> Durante a estimulação de schaffer colaterais é descrito. O isolamento farmacológica de potássio astroglial e as correntes do transportador de glutamato é demonstrada.
Tags
Dual Electrophysiological RecordingsSynaptically-evoked Astroglial ResponsesNeuronal ResponsesAcute Hippocampal SlicesTripartite SynapsesAstrocytesNeuronal Activity ModulationIon ChannelsNeurotransmitter ReceptorsGliotransmittersGlutamate UptakePotassium Spatial BufferingGABA ClearanceSynaptic Activity MonitoringAlterations In Glutamate LevelsAlterations In GABA LevelsAlterations In Extracellular Potassium LevelsAstroglial Uptake ActivityBuffering CapacityPhysiopathological SituationsKnockout MiceHippocampal Slice PreparationStratum Radiatum Astrocytes IdentificationSimultaneous Neuronal And Astroglial RecordingsSynaptically-evoked Astroglial Currents Isolation
