JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Biology
/
从长期来看,高解析度激光共聚焦时间流逝成像<em>拟南芥子叶</em>表皮萌发过程中
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Long-term, High-resolution Confocal Time Lapse Imaging of
Arabidopsis Cotyledon
Epidermis during Germination
Please note that all translations are automatically generated.
Click here for the English version.
从长期来看,高解析度激光共聚焦时间流逝成像<em>拟南芥子叶</em>表皮萌发过程中
DOI:
10.3791/4426-v
•
12:01 min
•
December 31, 2012
•
Kylee M. Peterson
,
Keiko U. Torii
2,3
1
Department of Biology
,
University of Washington
,
2
Howard Hughes Medical Institute
,
University of Washington
,
3
PRESTO
,
Japan Science and Technology Agency
Chapters
00:05
Title
01:00
Seed Sterilization
01:57
Preparation of Chamber Slide
03:11
Seed Dissection
04:47
Mounting Cotyledons in the Chamber Slide
05:40
Time Lapse Imaging
09:29
Results: Changes in Localization of POLAR Protein during Proliferation Stage of Stomatal-lineage Cells in the Cotyledon Epidermis
11:40
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们描述了一个协议,用玻片和媒体的固定几天的发展,记录植物子叶共聚焦成像的表皮气孔分化。萤光标记的蛋白质,可以动态跟踪的表达和亚细胞定位,细胞分裂和细胞分化过程中增进了解其可能的作用。
Tags
Confocal Time Lapse Imaging
Arabidopsis Cotyledon
Cellular Behavior
In Vivo Dynamics
Plant Development
Long-term Live Imaging
Cell Division
Differentiation Events
Arabidopsis Epidermis
Signaling
Cell Fate
Stomata
Asymmetric Division
Cell Differentiation Steps
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
长期使用宽视场显微镜的成像哺乳动物细胞
双光子现场斑马鱼的胚胎干切断和时间推移共焦成像
有丝分裂的siRNA转染后的时间推移成像
颅面部发育中的SOX10可视化:使用枫转基因斑马鱼线时间推移共聚焦显微镜
使用平板扫描仪,以收集拟南芥根向地反应的高分辨率时间失效图片
高分辨率成像定时,并在活体人脐静脉内皮细胞微管动力学自动分析
实时
在体
记录了
拟南芥
钙信号在使用荧光传感器的昆虫饲喂时
有丝分裂细胞同步与高分辨共聚焦显微镜联合应用研究细胞周期蛋白在有丝分裂过程中的作用
一种用于阴
南芥
叶片安装的通用方法
延时结构照明显微镜线粒体核素的特定标签
Read Article