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Super-resolution Imaging of the Bacterial Division Machinery
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JoVE Journal Biology
Super-resolution Imaging of the Bacterial Division Machinery

Super-resolution Imaging of the Bacterial Division Machinery

DOI:
10.3791/50048-v

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08:47 min

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January 21, 2013

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, ,
1Department of Biophysics and Biophysical Chemistry,The Johns Hopkins University School of Medicine

Chapters

  • 00:05Title
  • 01:21Sample Preparation and Assembly of the Image Chamber
  • 03:38Image Acquisition
  • 05:30Photo-activated Localization Microscopy (PALM) Image Construction
  • 06:42Results: Structural Measurements from PALM Images
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a super-resolution imaging method to probe the structural organization of the bacterial FtsZ-ring, an essential apparatus for cell division. This method is based on quantitative analyses of photoactivated localization microscopy (PALM) images and can be applied to other bacterial cytoskeletal proteins.

Tags

Keywords: Super-resolution ImagingBacterial Division MachineryFtsZ ProteinZ-ringProtofilamentsPhotoactivated Localization Microscopy (PALM)E. Coli

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