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超分辨率成像细菌科机械
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Biology
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JoVE Journal
Biology
Super-resolution Imaging of the Bacterial Division Machinery
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超分辨率成像细菌科机械
DOI:
10.3791/50048-v
•
08:47 min
•
January 21, 2013
•
Jackson Buss
,
Carla Coltharp
,
Jie Xiao
1
Department of Biophysics and Biophysical Chemistry
,
The Johns Hopkins University School of Medicine
Chapters
00:05
Title
01:21
Sample Preparation and Assembly of the Image Chamber
03:38
Image Acquisition
05:30
Photo-activated Localization Microscopy (PALM) Image Construction
06:42
Results: Structural Measurements from PALM Images
08:14
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了超分辨率成像方法的细菌FtsZ的环探测的组织结构,细胞分裂的重要设备。这种方法是基于光活化的定位显微镜(PALM)图像的定量分析,并可以应用到其他的细菌的细胞骨架蛋白。
Tags
Super-resolution Imaging
Bacterial Division Machinery
FtsZ Protein
Z-ring
Protofilaments
Photoactivated Localization Microscopy (PALM)
E. Coli
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