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产生和回收的β-细胞的细胞球体从逐步增长的PEG-肽水凝胶
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels
DOI:

09:21 min

December 06, 2012

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Chapters

  • 00:05Title
  • 02:01Material Preparation and Sterilization
  • 03:26Cell Preparation
  • 04:46Hydrogel Fabrication and Cell Encapsulation
  • 05:57Chymotrypsin Mediated Gel Erosion and Spheroid Recovery
  • 07:24Results: Spheroid Encapsulation, Survival, Proliferation, and Recovery in Thiol-ene Hydrogels
  • 08:31Conclusion

Summary

Automatic Translation

下面的协议提供了用于封装在逐步增长的PEG-肽水凝胶形成的硫醇 - 烯照片点击反应胰腺β-细胞的技术。这种材料平台不仅提供了细胞相容性的微环境,细胞封装,但也允许用户控制的快速恢复细胞结构内形成的水凝胶。

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