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Cryosectioning Gær Fællesskaber for Undersøgelse Fluorescens Patterns
JoVE Journal
Biology
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JoVE Journal Biology
Cryosectioning Yeast Communities for Examining Fluorescence Patterns
DOI:

07:03 min

December 26, 2012

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Chapters

  • 00:05Title
  • 00:58Fixing Yeast Communities
  • 03:40Sectioning Frozen Communities for Fluorescence Imaging
  • 05:15Results: Fluorescence Images of Vertical Cross-sections of Yeast Communities
  • 06:44Conclusion

Summary

Automatic Translation

Vi præsenterer en protokol til frysning og cryosectioning gær samfund til at observere interne mønstre af fluorescerende celler. Metoden bygger på methanol-fixing og OLT-indlejring for at bevare den rumlige fordeling af celler uden at inaktivere fluorescerende proteiner inden for et fællesskab.

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