JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
简单和高效的睾丸细胞悬液的制备技术
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
Please note that all translations are automatically generated.
Click here for the English version.
简单和高效的睾丸细胞悬液的制备技术
DOI:
10.3791/50102-v
•
15:33 min
•
August 04, 2013
•
Rosana Rodríguez-Casuriaga
,
Gustavo A. Folle
,
Federico Santiñaque
,
Beatriz López-Carro
,
Adriana Geisinger
3
1
Departamento de Biología Molecular
,
Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)
,
2
Servicio de Citometría de Flujo y Clasificación Celular (SECIF)
,
Instituto de Investigaciones Biológicas Clemente Estable
,
3
Sección Bioquímica, Facultad de Ciencias
,
Universidad de la República
Chapters
00:00
Title
02:02
Preparation of the Cellular Suspension
04:55
Flow Cytometric Analysis
08:29
Results
14:22
Conclusions
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
睾丸细胞悬浮液从啮齿动物材料，避免酶和洗涤剂，机械制备一种新的协议进行说明。方法很简单，速度快，重复性好，并呈现良好的品质，这是适合流式分选和RNA提取的细胞悬液。
Tags
Testicular Cell Suspension
Mammalian Spermatogenesis
Cell Enrichment
Cell Purification
Mechanical Disaggregation
Flow Cytometry
Gene Expression Studies
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
gentleMACS Dissociator从小鼠脾脏单细胞悬浮液的制备
标准化的制备小鼠肺组织单细胞悬浮液使用gentleMACS Dissociator
从小鼠神经组织产生单细胞悬浮液
汇集人类血小板裂解液作为一种有效的补充（pHPL）的动物血清自由人干细胞培养制备
电子显微镜对胚胎的制备
果蝇
胚胎心管
编制质量肌醇Pyrophosphates
液泡和胞质pH值的测量<em>在体内</em在酵母细胞悬浮液
高级别瞬态表达重组蛋白的植物高效的农杆菌
从血液高效的iPS细胞生成使用附加体和HDAC抑制剂
高效的哺乳动物细胞表达和细胞外糖蛋白的结晶一步纯化
Read Article
