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简单和高效的睾丸细胞悬液的制备技术
JoVE Journal
Biology
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JoVE Journal Biology
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
DOI:

15:33 min

August 04, 2013

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Chapters

  • 00:00Title
  • 02:02Preparation of the Cellular Suspension
  • 04:55Flow Cytometric Analysis
  • 08:29Results
  • 14:22Conclusions

Summary

Automatic Translation

睾丸细胞悬浮液从啮齿动物材料,避免酶和洗涤剂,机械制备一种新的协议进行说明。方法很简单,速度快,重复性好,并呈现良好的品质,这是适合流式分选和RNA提取的细胞悬液。

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