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JoVE Journal
Biology

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基因捕获斑马鱼中使用的Gal4
 
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基因捕获斑马鱼中使用的Gal4

Article DOI: 10.3791/50113
September 29th, 2013

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Summary September 29th, 2013

Please note that all translations are automatically generated.

Click here for the English version.

本协议描述的使用GAL4-VP16为主要记者和GFP / RFP在斑马鱼次要记者基因陷阱插入突变的方法。约于一体的高表达F0鱼产量的基因陷阱后代共同表达GFP和RFP十名。筛选过程可以容易地缩放,以适应执行插入诱变屏幕实验室的规模。

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