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Silencing a lungo termine di Intersectin-1s in Polmoni mouse consegna ripetuta di un siRNA specifico tramite liposomi cationici. Valutazione degli effetti Knockdown al microscopio elettronico
JoVE Journal
Biology
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JoVE Journal Biology
Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
DOI:

15:55 min

June 21, 2013

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Chapters

  • 00:05Title
  • 02:06Prepare Cationic Liposomes and Liposomes:siRNA-ITSN-1 Complexes
  • 05:30Mouse Retro-orbital Vein Injection (Internal Angle of the Eye Socket)
  • 06:39Mouse Lung Perfusion and Tissue Collection for Biochemical Studies
  • 08:23Mouse Lung Tissue Processing for EM
  • 10:21Results: Down-regulation of Intersectin-1 Expression Affects Morphology of Lung Tissue
  • 15:14Conclusion

Summary

Automatic Translation

Ripetute iniezioni retro-orbitali di liposomes/siRNA/ITSN-1s cationici complessi nei topi, ogni 72 ore per 24 giorni, fornire in modo efficiente il duplex siRNA per il mouse microcircolo polmonare riducendo mRNA ITSN-1s e di espressione proteica del 75%. Questa tecnica è altamente riproducibile in un modello animale, non ha effetti collaterali ed evita infortuni mortali.

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