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Immunology and Infection
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肽为基础的识别功能图案和具有约束力的合作伙伴
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Peptide-based Identification of Functional Motifs and their Binding Partners
Please note that all translations are automatically generated.
Click here for the English version.
肽为基础的识别功能图案和具有约束力的合作伙伴
DOI:
10.3791/50362-v
•
14:28 min
•
June 30, 2013
•
Martin N. Shelton
2
,
Ming Bo Huang
,
Syed Ali
3
,
Kateena Johnson
,
William Roth
,
Michael Powell
,
Vincent Bond
1
Department of Microbiology, Biochemistry, & Immunology
,
Morehouse School of Medicine
,
2
Institute for Systems Biology
,
3
Advanced Medical & Dental Institute
,
Universiti Sains Malaysia
Chapters
00:05
Title
02:55
Nef Scanning Peptide TUNEL Assay
04:43
Competitive Inhibition Analysis Using Peptides
06:02
Isolating Motif Binding Partners Using Peptides as Bait
09:25
Nef-GFP Secretion Fluorescent Plate Reader Assay of Antibody Inhibition
10:33
Identification of Nef Functional Motifs and their Binding Partners
13:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
解剖机制的分泌HIV-1 Nef的外来技术进行了阐述。来自NEF和蛋白转染的特殊短肽被利用来确定Nef的分泌修改区域的结构,功能和有约束力的合作伙伴。这些程序在许多机理研究具有普遍意义。
Tags
Peptide
Motif
Binding Partner
HIV-1 Nef
Apoptosis
Secretion Modification Region (SMR)
Exosome
Mortalin
Inhibitor
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