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Journal / Immunology and Infection / Peptid-baserede identifikation af…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Peptid-baserede identifikation af funktionskravene motiver og deres bindingspartnere
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Viral Infection of Human Intestinal Organoid Monolayers for Live Fluorescence Imaging
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Peptid-baserede identifikation af funktionskravene motiver og deres bindingspartnere

Article DOI: 10.3791/50362-v • 14:28 min • June 30th, 2013
June 30th, 2013 •
1,2, 1, 1,3, 1, 1, 1, 1
1Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute, Universiti Sains Malaysia

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Teknikker til at dissekere mekanismerne bag udskillelsen af ​​HIV-1 Nef i exosomes beskrevet. Specifikke korte peptider afledt fra Nef og protein transfektion blev udnyttet til at bestemme strukturen, funktionen og bindingspartnere af Nef s Sekretion Ændring Region. Disse procedurer har generel relevans i mange mekanistiske undersøgelser.

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Virology Biokemi Immunologi infektion smitsomme sygdomme molekylær biologi medicin genetik mikrobiologi Genomics proteiner exosomer HIV peptider exocytose protein menneskesmugling sekretion HIV-1 Nef Sekretion Ændring Region SMR peptid AIDS assay
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