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Journal / Immunology and Infection / Peptide-baserte Identifikasjon av Funksjonelle…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Peptide-baserte Identifikasjon av Funksjonelle Motifs og deres Binding Partnere
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Peptide-baserte Identifikasjon av Funksjonelle Motifs og deres Binding Partnere

Article DOI: 10.3791/50362-v • 14:28 min • June 30th, 2013
June 30th, 2013 •
1,2, 1, 1,3, 1, 1, 1, 1
1Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute, Universiti Sains Malaysia

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Teknikker for å dissekere mekanismene bak utskillelsen av HIV-1 Nef i exosomes er beskrevet. Spesifikke korte peptider avledet fra Nef protein transfeksjon ble utnyttet for å bestemme strukturen, funksjonen og bindingspartnere av Nef Sekresjon Modification Region. Disse prosedyrene har generell relevans i mange mekanistiske studier.

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Virologi biokjemi immunologi infeksjon Infectious Diseases molekylærbiologi medisin genetikk mikrobiologi genomikk proteiner Exosomes HIV peptider exocytosis protein trafficking sekresjon HIV-1 NEF sekresjon Modification Region SMR peptid AIDS analysen
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