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Journal / Immunology and Infection / Peptid-baserad identifiering av funktionella…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Peptid-baserad identifiering av funktionella motiv och deras bindningspartners
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Peptid-baserad identifiering av funktionella motiv och deras bindningspartners

Article DOI: 10.3791/50362-v • 14:28 min • June 30th, 2013
June 30th, 2013 •
1,2, 1, 1,3, 1, 1, 1, 1
1Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute, Universiti Sains Malaysia

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Tekniker för att dissekera mekanismerna bakom utsöndringen av HIV-1 Nef i exosomes beskrivs. Specifika korta peptider härledda från Nef och protein transfektion utnyttjades för att bestämma struktur, funktion och bindande partner Nef utsöndring Modification Region. Dessa förfaranden har allmän relevans i många mekanistiska studier.

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Virologi biokemi immunologi infektion Infektionskliniken molekylärbiologi medicin genetik mikrobiologi genomik proteiner exosomes HIV peptider exocytosis protein trafficking sekretion HIV-1 Nef Secretion Ändring Region SMR peptid aids analys
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