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Immunology and Infection
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Isolation, Processing and Analysis of Murine Gingival Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation, Processing and Analysis of Murine Gingival Cells

Isolation, Processing and Analysis of Murine Gingival Cells

DOI:
10.3791/50388-v

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09:47 min

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July 02, 2013

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, , ,
1Institute of Dental Sciences,Hebrew University – Hadassah Medical Center, 2Department of Periodontology,Hebrew University – Hadassah Medical Center

Chapters

  • 00:05Title
  • 01:49Upper Gingival Excision
  • 03:24Gingival Processing
  • 04:57Extracellular and Intracellular Antibody Staining for Flow Cytometry Analysis
  • 06:58Representative Gingival Cell Analysis
  • 08:40Conclusion

Summary

Automatic Translation

Automatic Translation

This study describes an efficient technique to isolate and process gingival tissues from the mouse oral cavity in order to produce a single-cell culture. The resulting cells can be further used for flow cytometry analysis and molecular studies.

Tags

Keywords: Murine Gingival CellsIsolationFlow CytometryMolecular StudiesImmune MechanismsPeriodontal DiseasesAlveolar BoneLangerhans CellsT Regulatory CellsOral Immune System

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