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Journal
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Immunology and Infection
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小鼠牙龈细胞的分离,处理和分析
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation, Processing and Analysis of Murine Gingival Cells
Please note that all translations are automatically generated.
Click here for the English version.
小鼠牙龈细胞的分离,处理和分析
DOI:
10.3791/50388-v
•
09:47 min
•
July 02, 2013
•
Gabriel Mizraji*
1,2
,
Hadas Segev*
1
,
Asaf Wilensky*
2
,
Avi-Hai Hovav*
1
1
Institute of Dental Sciences
,
Hebrew University – Hadassah Medical Center
,
2
Department of Periodontology
,
Hebrew University – Hadassah Medical Center
Chapters
00:05
Title
01:49
Upper Gingival Excision
03:24
Gingival Processing
04:57
Extracellular and Intracellular Antibody Staining for Flow Cytometry Analysis
06:58
Representative Gingival Cell Analysis
08:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究中描述了一种有效的技术,用于隔离和处理,以产生一个单一的细胞培养的小鼠口腔牙龈组织。得到的细胞可进一步用于流式细胞分析和分子水平的研究。
Tags
Murine Gingival Cells
Isolation
Flow Cytometry
Molecular Studies
Immune Mechanisms
Periodontal Diseases
Alveolar Bone
Langerhans Cells
T Regulatory Cells
Oral Immune System
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