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在小鼠视网膜神经细胞,实现动态夹具与突触和人工电导
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
DOI:

11:46 min

May 16, 2013

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Chapters

  • 00:05Title
  • 02:05General Set Up and Tissue Preparation
  • 05:32Patching Cell Bodies of Retinal Ganglion Cells
  • 07:20Recordings of Ganglion Cells Using Dynamic Clamp
  • 09:12Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells
  • 10:14Results: Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
  • 11:27Conclusion

Summary

Automatic Translation

此视频文章说明设置,程序,修补细胞组织,以及如何实现动态钳记录整个安装鼠标视网膜神经节细胞的。这种技术允许调查的兴奋性和抑制性突触输入精确的贡献,他们的相对幅度和时机神经扣球。

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