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在小鼠视网膜神经细胞，实现动态夹具与突触和人工电导
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JoVE Journal Neuroscience
Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
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在小鼠视网膜神经细胞，实现动态夹具与突触和人工电导

DOI:
10.3791/50400-v

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11:46 min

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May 16, 2013

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, ,
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney , 2The MARCS Institute,University of Western Sydney, 3Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney

Chapters

  • 00:05Title
  • 02:05General Set Up and Tissue Preparation
  • 05:32Patching Cell Bodies of Retinal Ganglion Cells
  • 07:20Recordings of Ganglion Cells Using Dynamic Clamp
  • 09:12Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells
  • 10:14Results: Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
  • 11:27Conclusion

Summary

Automatic Translation

Automatic Translation

此视频文章说明设置，程序，修补细胞组织，以及如何实现动态钳记录整个安装鼠标视网膜神经节细胞的。这种技术允许调查的兴奋性和抑制性突触输入精确的贡献，他们的相对幅度和时机神经扣球。

Tags

Dynamic ClampSynaptic ConductanceArtificial ConductanceRetinal Ganglion CellsPatch ClampVoltage ClampCurrent ClampNeuronal ExcitabilityExcitatory InputsInhibitory InputsAlpha Functions

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