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Neuroscience
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在小鼠视网膜神经细胞,实现动态夹具与突触和人工电导
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
Please note that all translations are automatically generated.
Click here for the English version.
在小鼠视网膜神经细胞,实现动态夹具与突触和人工电导
DOI:
10.3791/50400-v
•
11:46 min
•
May 16, 2013
•
Jin Y. Huang
,
Klaus M. Stiefel
,
Dario A. Protti
1
Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute
,
University of Sydney
,
2
The MARCS Institute
,
University of Western Sydney
,
3
Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute
,
University of Sydney
Chapters
00:05
Title
02:05
General Set Up and Tissue Preparation
05:32
Patching Cell Bodies of Retinal Ganglion Cells
07:20
Recordings of Ganglion Cells Using Dynamic Clamp
09:12
Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells
10:14
Results: Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
11:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此视频文章说明设置,程序,修补细胞组织,以及如何实现动态钳记录整个安装鼠标视网膜神经节细胞的。这种技术允许调查的兴奋性和抑制性突触输入精确的贡献,他们的相对幅度和时机神经扣球。
Tags
Dynamic Clamp
Synaptic Conductance
Artificial Conductance
Retinal Ganglion Cells
Patch Clamp
Voltage Clamp
Current Clamp
Neuronal Excitability
Excitatory Inputs
Inhibitory Inputs
Alpha Functions
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