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Gennemførelse Dynamic Clamp med Synaptic og Artificial konduktanser i Mus retina-ganglieceller
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Neuroscience
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JoVE Journal Neuroscience
Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

Gennemførelse Dynamic Clamp med Synaptic og Artificial konduktanser i Mus retina-ganglieceller

DOI:
10.3791/50400-v

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11:46 min

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May 16, 2013

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, ,
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney , 2The MARCS Institute,University of Western Sydney, 3Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney

Chapters

  • 00:05Title
  • 02:05General Set Up and Tissue Preparation
  • 05:32Patching Cell Bodies of Retinal Ganglion Cells
  • 07:20Recordings of Ganglion Cells Using Dynamic Clamp
  • 09:12Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells
  • 10:14Results: Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
  • 11:27Conclusion

Summary

Automatic Translation

Automatic Translation

Denne video artikel illustrerer set-up, de procedurer til at lappe celle organer og hvordan man gennemfører dynamiske clamp optagelser fra ganglion celler i hel-mount mus retinae. Denne teknik gør det muligt at undersøge den præcise bidrag excitatoriske og hæmmende synaptiske input, og deres relative størrelse og timing at neuronal spiking.

Tags

Dynamic ClampSynaptic ConductanceArtificial ConductanceRetinal Ganglion CellsPatch ClampVoltage ClampCurrent ClampNeuronal ExcitabilityExcitatory InputsInhibitory InputsAlpha Functions

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