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Chapters
- 00:05Title
- 02:40Amplification of the Template
- 04:01Vacuum Workstation Protocol
- 04:05Immobilizing the Biotinylated PCR Products
- 05:06Denaturation of DNA and Addition of Sequencing Primer
- 07:30Annealing Sequencing Primer to DNA Strands
- 07:55Pyromark Q24 Operation for Pyrosequencing
- 07:58Preparing for a Run
- 08:58Starting a Run
- 09:46Completing a Run
- 10:03Results: Pyrosequencing Identifies Bacteria and Detects Antibiotic Drug Resistance
- 11:35Conclusion
Pyrosequencing è una tecnica versatile che facilita microbica sequenziamento del genoma che può essere utilizzato per identificare le specie batteriche, discriminare ceppi batterici, e rilevare mutazioni genetiche che conferiscono resistenza agli agenti antimicrobici. In questo video, verrà dimostrata la procedura per la generazione microbica amplicone, amplicone pyrosequencing, e l'analisi di sequenza del DNA.
Tags
Keywords:
PyrosequencingMicrobial IdentificationMicrobial CharacterizationBacterial Species IdentificationBacterial Strain DiscriminationAntimicrobial Resistance DetectionHigh-throughput ScreeningMicrobial Genome SequencingSingle Base SequencingChemiluminescent DetectionReal-time SequencingSNP TypingMutation Detection
