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Genetisk Manipulation af musen Udvikling Hypothalamus gennem<em> In Utero</em> Elektroporering
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Neuroscience
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JoVE Journal Neuroscience
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Genetisk Manipulation af musen Udvikling Hypothalamus gennem<em> In Utero</em> Elektroporering

DOI:
10.3791/50412-v

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11:48 min

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July 24, 2013

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, , ,
1Institute of Anatomy and Cell Biology,University of Heidelberg , 2Institut de recherches cliniques de Montreal

Chapters

  • 00:05Title
  • 02:06Preparation of DNA and Glass Micropipettes for Injection
  • 02:57Laparotomy
  • 04:33DNA Injection into the Brain Ventricle
  • 05:59Targeting the Hypothalamus for Electroporation
  • 07:17Finishing the Surgery
  • 08:28Analyzing the Results
  • 09:18Results: Hypothalamus Transfection by In utero Electroporation
  • 10:54Conclusion

Summary

Automatic Translation

Automatic Translation

Trods den funktionelle og medicinske betydning af hypothalamus,<em> In utero</em> Genmanipulation af sin udvikling er sjældent blevet forsøgt. Vi viser en detaljeret procedure for<em> In utero</em> Elektroporation i musen hypothalamus og viser repræsentative resultater af total og delvis (regional) hypothalamus transfektion.

Tags

Genetic ManipulationMouseDeveloping HypothalamusIn Utero ElectroporationGenetic ModificationMammalian BrainMouse MutantsDeveloping BrainCortexThalamusHypothalamusHypothalamic NeuroepitheliumMammillary BodyElectroporation

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