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人类心肌细胞的生成:从馈线人类诱导多能干细胞的分化协议
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Biology
Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
人类心肌细胞的生成:从馈线人类诱导多能干细胞的分化协议
DOI:
10.3791/50429-v
•
13:18 min
•
June 28, 2013
•
Elisa Di Pasquale
2
,
Belle Song
,
Gianluigi Condorelli
1
Humanitas Clinical and Research Center, Italy
,
2
Institute of Genetic and Biomedical Research (IRGB)
,
National Research Council (CNR)
Chapters
00:05
Title
02:15
Feeder-free Maintenance and Passaging of Human iPS Cell Lines
03:54
Embryoid Bodies Aggregation and Cardiac Induction
06:25
Beating Areas Isolation and Culture
07:48
Single Beating Cells Isolation and Analysis
10:06
Characterizing Contracting Cardiomyocytes Derived from iPS Cells
12:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
多能干细胞,胚胎或诱导多能干细胞(iPS),构成人类分化的细胞,包括心肌细胞的重要来源。在这里,我们将专注于心脏iPS细胞的诱导,展示如何使用它们来获得功能的人类心肌细胞通过胚状体为基础的协议。
Tags
Human Cardiomyocytes
Induced Pluripotent Stem Cells
Differentiation Protocol
Feeder-free
Heart Development
Myocardial Diseases
Drug Discovery
Toxicology
Cardiac Disorders
Embryoid Bodies
Chemically Defined Protocols
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