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Generation of Human Cardiomyocytes: En Differensiering Protokoll fra mater-frie menneskeskapte pluripotent stamceller
JoVE Journal
Biology
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JoVE Journal Biology
Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
DOI:

13:18 min

June 28, 2013

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Chapters

  • 00:05Title
  • 02:15Feeder-free Maintenance and Passaging of Human iPS Cell Lines
  • 03:54Embryoid Bodies Aggregation and Cardiac Induction
  • 06:25Beating Areas Isolation and Culture
  • 07:48Single Beating Cells Isolation and Analysis
  • 10:06Characterizing Contracting Cardiomyocytes Derived from iPS Cells
  • 12:36Conclusion

Summary

Automatic Translation

Pluripotente stamceller, enten embryonale eller indusert pluripotente stamceller (iPS) celler, utgjør en verdifull kilde til menneskelige differensierte celler, inkludert cardiomyocytes. Her vil vi fokusere på hjertestans induksjon av iPS-celler, som viser hvordan du bruker dem til å oppnå funksjonelle menneskelige cardiomyocytes gjennom en embryoid organer-basert protokoll.

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