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神经元的分化的人类Neuroprogenitor细胞培养激光捕获显微切割
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Neuroscience
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JoVE Journal Neuroscience
Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
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神经元的分化的人类Neuroprogenitor细胞培养激光捕获显微切割

DOI:
10.3791/50487-v

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12:38 min

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September 16, 2013

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, ,
1Section of Endocrinology,Denver VA Medical Center, 2Department of Medicine,University of Colorado Denver School of Medicine

Chapters

  • 00:00Title
  • 01:51Expansion and Differentiation of Human Neuroprogenitor Cells
  • 05:28Immunofluorescence Staining of Differentiated Neuroprogenitor
  • 10:17Results: Laser Capture Microdissection of Differrntiated Neurons
  • 12:14Conclusion

Summary

Automatic Translation

Automatic Translation

人类neuroprogenitor细胞（的NPC）增殖的条件下扩大。的NPC分化成神经营养因子中的组合的存在下神经元的富培养物。用免疫组化法检测神经元标志物。为了分离神经元的纯人口的NPC分化的PEN膜幻灯片并进行激光捕获显微切割。

Tags

Laser Capture MicrodissectionNeuronsDifferentiated Human Neuroprogenitor CellsCell CultureNeuronal MarkersAstrocyte MarkersNeuNSynapsinAcetylcholinesteraseSynaptophysinGAP43GFAPSTAT3Molecular Characterization

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