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Laser Capture Microdissection av nervceller från Differentierad Human Neuroprogenitor Celler i kultur
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
DOI:

12:38 min

September 16, 2013

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Chapters

  • 00:00Title
  • 01:51Expansion and Differentiation of Human Neuroprogenitor Cells
  • 05:28Immunofluorescence Staining of Differentiated Neuroprogenitor
  • 10:17Results: Laser Capture Microdissection of Differrntiated Neurons
  • 12:14Conclusion

Summary

Automatic Translation

Mänskliga neuroprogenitor celler (NPC) expanderades enligt prolifererande betingelser. NPC var differentierade i neuron-rika kulturer i närvaro av en kombination av neurotrofiner. Neuronala markörer detekterades genom immunofluorescens-färgning. För att isolera en ren population av nervceller, var NPCs differentieras på PEN-membran diabilder och laser capture microdissection utfördes.

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