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使用人类肠道上皮细胞和BALB / C小鼠巨噬细胞的容忍脂肪酸和霍乱毒素浓度的测定
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
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使用人类肠道上皮细胞和BALB / C小鼠巨噬细胞的容忍脂肪酸和霍乱毒素浓度的测定

DOI:
10.3791/50491-v

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09:39 min

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May 30, 2013

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, ,
1Department of Biological Sciences,Kingsborough Community College, 2Institute for Cellular and Molecular Biology,University of Texas at Austin, 3New Jersey Center for Science, Technology and Mathematics,Kean University

Chapters

  • 00:05Title
  • 00:59Tissue Culture
  • 02:24Fatty Acid Treatment
  • 04:12Cholera Toxin Treatment
  • 05:38MTT Assay
  • 06:47Results: Effects of Fatty Acids and Cholera Toxin on Cell Survival
  • 09:16Conclusion

Summary

Automatic Translation

Automatic Translation

我们容许浓度的三脂肪酸（油酸，亚油酸和亚麻酸）和霍乱毒素没有显着不利影响细胞生存脂肪酸和毒素溶解，并利用他们在细胞存活实验来确定。

Tags

Fatty AcidsCholera ToxinHuman Intestinal Epithelial CellsBALB/c Mouse MacrophagesCell Proliferation AssaysMucosal ImmunityCholera Prevention

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