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JoVE Journal Biology
Eine quantitative Assay zur Protein Studie
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Eine quantitative Assay zur Protein Studie: DNA-Wechselwirkungen, Discover Transkriptionsregulatoren der Genexpression, und identifizieren Novel Anti-Tumor-Wirkstoffe

Article DOI: 10.3791/50512-v • 06:43 min • August 31st, 2013
August 31st, 2013 •
1, 1, 2, 3, 4, 3,5
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

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Wir entwickelten eine quantitative DNA-bindenden, ELISA-basierten Assay Transkriptionsfaktor-Interaktionen mit DNA zu messen. Hohe Spezifität für das RUNX2 Protein wurde mit einer Consensus-DNA-Oligonukleotid und Erkennung spezifischer monoklonaler Antikörper erreicht. Kolorimetrischen Nachweis mit einem Enzym-gekoppelten Antikörper Substrat-Reaktion wurde in Echtzeit überwacht.

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Cellular Biology Ausgabe 78 Transkriptionsfaktoren Vitamin D Drug Discovery Enzyme-Linked Immunosorbent Assay (ELISA) DNA-bindende Transkriptionsfaktor Drogen-Screening Antikörper
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