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Journal / Biology / En kvantitativ analys för att studera protein:…
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JoVE Journal Biology
En kvantitativ analys för att studera protein
Germ-Free Wasp Embryo Collection and Rearing with Nasonia-Rearing Medium (NRM) (Video) | JoVE
Journal (Biology)
Germ-Free Wasp Embryo Collection and Rearing with Nasonia-Rearing Medium (NRM) (Video) | JoVE
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
Journal (Biology)
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
 
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En kvantitativ analys för att studera protein: DNA-interaktioner, Discover transkriptionsregulatorer av genuttryck, och identifiera nya antitumörmedel

Article DOI: 10.3791/50512-v • 06:43 min • August 31st, 2013
August 31st, 2013 •
1, 1, 2, 3, 4, 3,5
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

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Vi utvecklade en kvantitativ DNA-bindning ELISA-baserad analys för att mäta transkriptionsfaktor interaktion med DNA. Hög specificitet för Runx2 protein uppnåddes med en konsensus-DNA-erkännande oligonukleotiden och specifik monoklonal antikropp. Kolorimetrisk detektion med en enzym-kopplad antikropp substratreaktionen övervakades i realtid.

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Cellulär Biology transkriptionsfaktorer vitamin D Drug Discovery enzymkopplad immunadsorberande analys (ELISA) DNA-bindande transkriptionsfaktor läkemedelsscreening antikropp
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