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定量检测,以研究蛋白质:DNA的相互作用,发现转录基因表达的调节,并确定新的抗肿瘤剂
JoVE Journal
Biology
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JoVE Journal Biology
A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
DOI:

06:43 min

August 31, 2013

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Chapters

  • 00:05Title
  • 01:49Cell Culture and Nuclear Protein Isolation
  • 02:46Preparation of 96-well Plates by Incubation with Nuclear Extracts
  • 03:58Measurement of RUNX2 DNA Binding
  • 05:36Results: Inhibition of RUNX2 DNA Binding
  • 06:22Conclusion

Summary

Automatic Translation

我们开发了一种定量的DNA结合,基于ELISA的测定法来测量与DNA的转录因子相互作用。高特异性的RUNX2蛋白与共识的DNA寡核苷酸识别和特异性单克隆抗体来实现。比色检测用酶偶联抗体底物反应的实时监测。

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