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JoVE Journal
Biology

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定量检测,以研究蛋白质:DNA的相互作用,发现转录基因表达的调节,并确定新的抗肿瘤剂
 
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定量检测,以研究蛋白质:DNA的相互作用,发现转录基因表达的调节,并确定新的抗肿瘤剂

Article doi: 10.3791/50512
August 31st, 2013

Summary August 31st, 2013

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我们开发了一种定量的DNA结合,基于ELISA的测定法来测量与DNA的转录因子相互作用。高特异性的RUNX2蛋白与共识的DNA寡核苷酸识别和特异性单克隆抗体来实现。比色检测用酶偶联抗体底物反应的实时监测。

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