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Flowcytometrische analyse van Bimoleculaire Fluorescentie Complementatie
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Flowcytometrische analyse van Bimoleculaire Fluorescentie Complementatie: Een High Throughput kwantitatieve methode om eiwit-eiwit interactie Studie

Article DOI: 10.3791/50529-v • 11:11 min • August 15th, 2013
August 15th, 2013 •
1, 1
1Department of Pharmacology, University of Illinois at Chicago

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Flowcytometrische analyse van Bimoleculaire Fluorescentie Complementatie biedt een hoge doorvoer kwantitatieve methode om eiwit-eiwit interacties te bestuderen. Deze methodologie kan worden toegepast op mapping eiwitbindingsplaatsen en screenen factoren die eiwit-eiwit interactie te bepalen.

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Molecular Biology biochemie celbiologie genetica farmacologie Proteins flowcytometrie Bimoleculaire Fluorescentie Complementatie BiFC kwantitatieve analyse eiwit-eiwit interacties Förster-energieoverdracht FRET Bioluminescence Resonance Energy Transfer BRET eiwit cellen transfectie fluorescentie microscopie
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