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Flowcytometrisk analyse av bimolecular Fluorescens complementation
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Flowcytometrisk analyse av bimolecular Fluorescens complementation: En høy gjennomstrømning Kvantitativ metode for å studere protein-protein interaksjon

Article DOI: 10.3791/50529-v • 11:11 min • August 15th, 2013
August 15th, 2013 •
1, 1
1Department of Pharmacology, University of Illinois at Chicago

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Flowcytometrisk analyse av bimolecular Fluorescens complementation gir en høy gjennomstrømming kvantitativ metode for å studere protein-protein interaksjon. Denne metoden kan brukes til kartlegging proteinbindingsseter og for screening av faktorer som regulerer protein-protein interaksjon.

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Molecular Biology biokjemi cellebiologi genetikk farmakologi Proteiner flowcytometrisystemer bimolecular Fluorescens complementation BiFC kvantitativ analyse protein-protein interaksjon Förster resonans energi overføring slite Bioluminescence Resonance Energy Transfer BRET protein celle transfeksjon fluorescens mikroskopi
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