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Flowcytometrisk analyse av bimolecular Fluorescens complementation: En høy gjennomstrømning Kvantitativ metode for å studere protein-protein interaksjon
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JoVE Journal Biology
Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flowcytometrisk analyse av bimolecular Fluorescens complementation: En høy gjennomstrømning Kvantitativ metode for å studere protein-protein interaksjon

DOI:
10.3791/50529-v

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11:11 min

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August 15, 2013

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,
1Department of Pharmacology,University of Illinois at Chicago

Chapters

  • 00:05Title
  • 02:05Cell Transfection
  • 03:46Cell Preparation for Flow Cytometry, Western Blot, and Immunofluorescence
  • 05:12Flow Cytometry and Immunofluorescence Microscopy
  • 06:50Data Analysis
  • 07:49Assessing AKAP-Lbc and PDE4D3 Interaction Using BiFC-Flow Cytometry
  • 10:17Conclusion

Summary

Automatic Translation

Automatic Translation

Flowcytometrisk analyse av bimolecular Fluorescens complementation gir en høy gjennomstrømming kvantitativ metode for å studere protein-protein interaksjon. Denne metoden kan brukes til kartlegging proteinbindingsseter og for screening av faktorer som regulerer protein-protein interaksjon.

Tags

Keywords: Flow CytometryBimolecular Fluorescence ComplementationBiFCProtein-protein InteractionAKAP-LbcPDE4D3Fluorescent ProteinHigh ThroughputQuantification

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