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代谢标记和膜分馏的比较蛋白质组学分析<em>拟南芥</em>悬浮细胞培养
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Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
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代谢标记和膜分馏的比较蛋白质组学分析<em>拟南芥</em>悬浮细胞培养

DOI:
10.3791/50535-v

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11:44 min

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September 28, 2013

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, ,
1Signaling Proteomics,Max Plank Institute of Molecular Plant Physiology, 2Department of Plant Systems Biology,University of Hohenheim

Chapters

  • 00:05Title
  • 01:35Plasma Membrane Purification
  • 03:56Detergent Resistant Membrane Preparation
  • 05:33Extraction of Proteins from the Detergent Resistant Fraction and In-solution Trypsin Digestion
  • 07:04Use of C18-StageTips for Peptide Desalting and Concentration
  • 08:48Results: Comparative Proteomic Analysis
  • 11:00Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了植物细胞质膜的基础上，未标记的混合物分馏成耐洗涤剂和清洁剂的可溶性和膜在体内完全15 N标记的拟南芥细胞培养一个强大的方法。该过程被施加于比较蛋白质组学研究，了解信令进程。

Tags

Metabolic LabelingMembrane FractionationComparative ProteomicsArabidopsis ThalianaSuspension Cell CulturesPlasma MembraneMicrodomainsDetergent-resistant Membrane FractionSucrose GradientQuantitative ProteomicsK15NO3 Labeling

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