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Måling vækst og genekspression Dynamics af Tumor-Målrettet<em> S. Typhimurium</em> Bakterier
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Growth and Gene Expression Dynamics of Tumor-Targeted S. Typhimurium Bacteria
DOI:

08:11 min

July 06, 2013

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Chapters

  • 00:01Title
  • 00:56Cell preparation and subcutaneous injection
  • 01:34Subcutaneous injection of cancer cells
  • 02:19Bacterial preparation for injection
  • 03:01Tail vein injection of bacteria
  • 03:44IVIS imaging of bacteria tumors
  • 04:11Organ dissection
  • 04:43Quantifying bacterial distribution in tumors
  • 05:08Discussion and Representative Results
  • 07:38Conclusions

Summary

Automatic Translation

Målet med disse eksperimenter er at generere kvantitative tidsforløb data på vækst og genekspression dynamik svækket<em> S. typhimurium</em> Bakteriekolonier vokser inde tumorer. Denne video tumorceller forberedelse og implantation, bakterier forberedelse og injektion, hel-animalsk luminescens billeddannelse, tumorexcision og bakteriekoloni tælling.

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