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活体显微镜成像的亚细胞结构的活体小鼠表达荧光蛋白
JoVE Journal
Biology
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JoVE Journal
Biology
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
Please note that all translations are automatically generated.
Click here for the English version.
活体显微镜成像的亚细胞结构的活体小鼠表达荧光蛋白
DOI:
10.3791/50558-v
•
08:23 min
•
September 01, 2013
•
Andrius Masedunskas
2
,
Natalie Porat-Shliom
,
Muhibullah Tora
,
Oleg Milberg
3
,
Roberto Weigert
1
Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch National Institute of Dental and Craniofacial Research
,
National Institutes of Health
,
2
Department of Biology
,
University of North Carolina at Chapel Hill
,
3
Department of Chemical & Biochemical Engineering and Department of Biomedical Engineering
,
Rutgers University
Chapters
00:05
Title
00:54
Animal Surgery and Positioning for Intravital Microscopy
04:34
Imaging Parameters
06:30
Results: Representative Intravital Microscopic Images of Salivary Gland Proteins
08:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
活体显微镜是一个功能强大的工具,使活体动物成像的各种生物过程。在本文中,我们提出了用于成像的亚细胞结构,如分泌颗粒,在活体小鼠的唾液腺的动力学的详细方法。
Tags
Intravital Microscopy
Live Mice
Fluorescent Proteins
Salivary Glands
Subcellular Structures
Exocytosis
Secretory Granules
Acinar Cells
Beta-adrenergic Receptors
GFP
Tandem-Tomato
Endosomes
Lysosomes
Mitochondria
Actin Cytoskeleton
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