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Chemistry
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隔离和脂质A的化学表征革兰氏阴性菌
JoVE Journal
Chemistry
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JoVE Journal
Chemistry
Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Please note that all translations are automatically generated.
Click here for the English version.
隔离和脂质A的化学表征革兰氏阴性菌
DOI:
10.3791/50623-v
•
12:57 min
•
September 16, 2013
•
Jeremy C. Henderson
,
John P. O’Brien
,
Jennifer S. Brodbelt
,
M. Stephen Trent
3
1
Section of Molecular Genetics and Microbiology
,
The University of Texas at Austin
,
2
Department of Chemistry and Biochemistry
,
The University of Texas at Austin
,
3
The Institute of Cellular and Molecular Biology
,
The University of Texas at Austin
Chapters
00:05
Title
02:00
Large Scale Lipid A Extraction (50 ml to 1.5 L)
06:22
Electrospray Ionization Mass Spectrometry of Lipid A and Collision Induced Dissociation
07:56
MS/MS on Lipid A by Ultraviolet Photodissociation (UVPD)
09:04
Results: Chemical Characterization of Lipid A by Mass Spectrometry and Thin Layer Chromatography
12:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
隔离脂多糖(LPS)由革兰氏阴性菌的类脂A结构域和表征提供了洞察抗生素耐药性的细胞表面为基础的机制,细菌的生存和健康,以及如何化学性质不同的脂质分子种类差异调节宿主先天免疫反应。
Tags
Lipopolysaccharide
Lipid A
Gram-negative Bacteria
Cell Surface Molecule
Chemical Characterization
Mass Spectrometry
Thin Layer Chromatography
Innate Immune Response
Antibiotic Resistance
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