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Isolation and Chemical Characterization of Lipid A fra Gram-negative bakterier
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
DOI:

12:57 min

September 16, 2013

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Chapters

  • 00:05Title
  • 02:00Large Scale Lipid A Extraction (50 ml to 1.5 L)
  • 06:22Electrospray Ionization Mass Spectrometry of Lipid A and Collision Induced Dissociation
  • 07:56MS/MS on Lipid A by Ultraviolet Photodissociation (UVPD)
  • 09:04Results: Chemical Characterization of Lipid A by Mass Spectrometry and Thin Layer Chromatography
  • 12:00Conclusion

Summary

Automatic Translation

Isolering og karakterisering av lipid Et domene av lipopolysakkarid (LPS) fra gramnegative bakterier gir innsikt i celleoverflatebaserte mekanismer for antibiotikaresistens, bakteriell overlevelse og fitness, og hvordan kjemisk mangfoldig lipid A molekylære arter differentially modulere vert medfødte immunresponser.

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