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Immunology and Infection

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培养小胶质细胞的新生和成年的中枢神经系统

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Immunology and Infection

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JoVE Journal Immunology and Infection

Culturing Microglia from the Neonatal and Adult Central Nervous System

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培养小胶质细胞的新生和成年的中枢神经系统

DOI:

10.3791/50647-v

•

11:28 min

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August 09, 2013

•

Robert Bronstein*^1,2, Luisa Torres*^2,3, Jillian C. Nissen*^2,3, Stella E. Tsirka

¹Program in Neuroscience,Stony Brook University, ²Department of Pharmacological Sciences,Stony Brook University, ³Program in Molecular and Cellular Pharmacology,Stony Brook University

Chapters

00:05Title
01:20Neonatal Microglia Dissection: Day 0
04:24Cell Culture: Day 0
05:31Cell Culture: Day 10
06:40Adult Microglia: Tissue Colection
07:56Digestion of Spinal Cord Tissue
09:07Results: Representative Images of Neonatal and Adult Microglia
10:50Conclusion

Summary

Automatic Translation

我们概述方法从新生儿大脑皮层和脊髓成人的可行的小胶质细胞的高效和快速隔离/文化。解剖和电镀皮质小胶质细胞可以在90分钟内完成，与随后发生〜10天的初始剥离后的小胶质细胞的收获。

Tags

Microglia CNS Central Nervous System Macrophage Neonatal Adult Multiple Sclerosis Spinal Cord Injury Axonal Damage Ischemic Brain Trauma Stroke Synaptic Plasticity Cell Culture Adult Cells Activation Pathology In Vitro

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