JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
生精细胞类型使用STA把速度沉降分离
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
Please note that all translations are automatically generated.
Click here for the English version.
生精细胞类型使用STA把速度沉降分离
DOI:
10.3791/50648-v
•
09:48 min
•
October 09, 2013
•
Jessica M Bryant
2
,
Mirella L Meyer-Ficca
,
Vanessa M Dang
,
Shelley L Berger
4,5
,
Ralph G Meyer
1
Department of Cell and Developmental Biology
,
University of Pennsylvania
,
2
Biomedical Graduate Studies
,
University of Pennsylvania
,
3
Department of Animal Biology and Center for Animal Transgenesis and Germ Cell Research
,
University of Pennsylvania
,
4
Department of Genetics
,
University of Pennsylvania
,
5
Department of Biology
,
University of Pennsylvania
Chapters
00:05
Title
01:07
STA-PUT Apparatus
03:50
Isolating Spermatogenic Cells from Whole Testes
05:28
Cell Loading and Sedimentation
08:04
Results: STA-PUT Purification of Testes
09:20
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在STA-PUT方法允许对生精细胞的不同人群根据大小和密度的分离。
Tags
Spermatogenic Cell Types
STA-PUT Velocity Sedimentation
Spermatogenesis
Testis Cell Types
Cell Isolation
Cell Enrichment
BSA Gradient
Sedimentation Velocity
Cell Yield
FACS
Elutriator
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
测量中使用FM标签的神经元的胞外分泌
肌动蛋白共沉淀测定; F -肌动蛋白结合蛋白分析
电泳分离蛋白质
交配和四分体分离
衣藻
遗传分析
两种类型的检测,检测青蛙精子Chemoattraction
琼脂糖凝胶电泳分离DNA片段
一种新型的全基因组分析的贝叶斯变点算法的多元ChIPseq数据类型
分离的小鼠胚胎面外胚层和间充质
激光辅助显微切割(LAM)作为个体细胞类型的转录谱的工具
通过热烫完整植株或提取物热处理烟草宿主细胞蛋白去除方法的比较
Read Article