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Journal
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Biology
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STA-PUTの速度沈降を使用して造精細胞型の分離
JoVE Journal
Biology
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JoVE Journal
Biology
Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
Please note that all translations are automatically generated.
Click here for the English version.
STA-PUTの速度沈降を使用して造精細胞型の分離
DOI:
10.3791/50648-v
•
09:48 min
•
October 09, 2013
•
Jessica M Bryant
2
,
Mirella L Meyer-Ficca
,
Vanessa M Dang
,
Shelley L Berger
4,5
,
Ralph G Meyer
1
Department of Cell and Developmental Biology
,
University of Pennsylvania
,
2
Biomedical Graduate Studies
,
University of Pennsylvania
,
3
Department of Animal Biology and Center for Animal Transgenesis and Germ Cell Research
,
University of Pennsylvania
,
4
Department of Genetics
,
University of Pennsylvania
,
5
Department of Biology
,
University of Pennsylvania
Chapters
00:05
Title
01:07
STA-PUT Apparatus
03:50
Isolating Spermatogenic Cells from Whole Testes
05:28
Cell Loading and Sedimentation
08:04
Results: STA-PUT Purification of Testes
09:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
STA-PUTメソッドは、大きさや密度に基づいて精子形成細胞の異なる集団の分離を可能にする。
Tags
Spermatogenic Cell Types
STA-PUT Velocity Sedimentation
Spermatogenesis
Testis Cell Types
Cell Isolation
Cell Enrichment
BSA Gradient
Sedimentation Velocity
Cell Yield
FACS
Elutriator
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